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Image Search Results
Journal: Journal of Virology
Article Title: Differential Vpu-Mediated CD4 and Tetherin Downregulation Functions among Major HIV-1 Group M Subtypes
doi: 10.1128/JVI.00293-20
Figure Lengend Snippet: Analysis of CD4 and tetherin downregulation function. (A) Representative flow cytometry plots demonstrating downregulation of CD4 (top) or tetherin (bottom) following transfection of a negative control (pSelect empty vector), a positive control (NL4.3 Vpu), a representative functional clone, and a representative nonfunctional clone. Gray-shaded areas define GFP-negative (untransfected) and the GFP-high (Vpu-expressing) gates used for analysis. The median fluorescence intensity (MFI) of receptor expression is indicated at the top of each gate, and the downregulation function value (calculated as described in Materials and Methods) normalized to NL4.3 is indicated at the lower right. The absolute values of CD4 and tetherin reduction by NL4.3 Vpu were 69% ± 14% and 73% ± 13%. (B) Normalized CD4 and tetherin downregulation results for the 332 Vpu clones assessed in this study. Data are reported as the mean from at least three independent experiments. Horizontal lines and values report the median and interquartile ranges. (C) Association between CD4 and tetherin downregulation function for all 332 Vpu clones. Correlation was evaluated using Spearman’s rank sum test.
Article Snippet: Second-round vpu amplicons were column purified using the E.Z.N.A Cycle Pure kit (Omega Bio-tek) and ligated into
Techniques: Flow Cytometry, Transfection, Negative Control, Plasmid Preparation, Positive Control, Functional Assay, Expressing, Fluorescence, Clone Assay